GSE29911

• Interferon (IFN) and pegylated interferon (PEG-IFN) treatment of chronic hepatitis B leads to a sustained virological response in a limited proportion of patients and has considerable side effects. 
• To find novel markers associated with prognosis of IFN therapy, we investigated whether a pretreatment plasma microRNA profile could be used to predict early virological response to IFN.

• The optimized model consisting of 11 microRNAs yielded a 74.2% overall accuracy in the training group and was independently confirmed in the test group (71.4% accuracy). 

• Univariate and multivariate logistic regression analyses confirmed its independent association with early virological response (OR=7.35; P=2.12×10(-5)). 

• Combining the microRNA profile with the alanine aminotransferase level improved the overall accuracy from 73.4% to 77.3%.

• Co-transfection of an HBV replicative construct with microRNA mimics revealed that let-7f, miR-939 and miR-638 were functionally associated with the HBV life cycle.

• A classification model has been built using Trainset. The selected probes were:
  1. hsa-mir-507
  2. hsa-mir-411
  3. hsa-mir-130b*
  4. hsa-mir-615-3p
  5. nc2_00106057

• The model has been tested using Testset.